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Updated: Aug 25, 2025

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
Published on: April 4, 2018
A new partial loss of function allele of rad-54.L
Chantal C Akerib1, Rayka Yokoo1, Emmanuel T Nsamba1
1Departments of Developmental Biology and Genetics, Stanford University School of Medicine, Stanford, CA 94305, U.S.A.
Abstract:
RAD-54.L is required for the repair of meiotic double-strand DNA breaks (DSBs), playing an essential role in promoting removal of recombinase RAD-51 and normal completion of meiotic recombination. Failure to complete meiotic DSB repair leads to 100% lethality of embryos produced by rad-54.L null mutant mothers. Here we report a new partial loss of function allele, rad-54.L(me139) , that may prove useful for investigating meiotic mechanisms by providing a sensitized genetic background that reduces but does not eliminate the essential functions of RAD-54.L.
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