Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intestinal microbial community assessment in patients with different forms of epilepsy and autoimmune encephalitis: An exploratory study.

Gut microbes reports·2026
Same author

The Lancet Commission on precision health: equitable, data-driven health outcomes for all.

Lancet (London, England)·2026
Same author

Dynamic structural and metabolic changes during the epileptogenesis in the pilocarpine model of temporal lobe epilepsy: A longitudinal MRI study.

Brain research·2026
Same author

Shared genetic architecture of brain age gap across 30 cohorts worldwide.

medRxiv : the preprint server for health sciences·2026
Same author

The HUGO Clinical Genomics & Genomic Medicine Education Survey: clinicians globally need and want genomic medicine training.

Human genomics·2025
Same author

Conceptualizing the public good for genomics in the global South: a cross-disciplinary roundtable dialogue.

Frontiers in genetics·2025

Related Experiment Video

Updated: Aug 25, 2025

Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD
08:29

Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD

Published on: October 10, 2012

16.2K

The transcriptome of rat hippocampal subfields.

João P D Machado1,2, Maria C P Athie3,2, Alexandre H B Matos3,2

  • 1Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.

IBRO Neuroscience Reports
|October 17, 2022
PubMed
Summary

This study maps unique molecular profiles of rat hippocampal subfields CA1, CA2, CA3, and dentate gyrus (DG) using laser capture microdissection and RNA-seq, revealing region-specific gene expression for distinct functions.

Keywords:
Hippocampal subfieldsHippocampusLaser-capture microdissectionRNA-SeqTranscriptomics

More Related Videos

Dissection of Hippocampal Dentate Gyrus from Adult Mouse
07:42

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Published on: November 17, 2009

83.0K
High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging
11:03

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

Published on: November 10, 2015

9.4K

Related Experiment Videos

Last Updated: Aug 25, 2025

Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD
08:29

Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD

Published on: October 10, 2012

16.2K
Dissection of Hippocampal Dentate Gyrus from Adult Mouse
07:42

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Published on: November 17, 2009

83.0K
High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging
11:03

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

Published on: November 10, 2015

9.4K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genomics

Background:

  • The hippocampus contains distinct neuronal populations (CA1, CA2, CA3, DG) with unique characteristics.
  • Previous RNA-seq studies provided a general hippocampal blueprint, but lacked region-specific data for rats.

Purpose of the Study:

  • To delineate the unique transcriptomic profiles of rat hippocampal subfields (CA1, CA2, CA3, DG).
  • To identify novel and known specific marker genes for each hippocampal region.
  • To understand the molecular basis of subfield-specific functions.

Main Methods:

  • Laser capture microdissection (LCM) was used to isolate specific hippocampal subfields from rat brain tissue.
  • RNA-sequencing (RNA-seq) was performed on isolated CA1, CA2, CA3, and DG samples.
  • Gene ontology enrichment analysis was conducted to interpret functional implications.

Main Results:

  • Transcriptomic profiles showed clear segregation between different hippocampal regions.
  • Specific marker genes, both known and novel, were identified for CA1, CA2, CA3, and DG.
  • CA1 was enriched for genes related to actin regulation and AMPA receptors (long-term potentiation).
  • CA2 and CA3 showed enrichment for metabolic processes.
  • DG exhibited enrichment for ribosome and spliceosome genes (protein synthesis).

Conclusions:

  • The study provides a detailed molecular map of rat hippocampal subfields.
  • Distinct molecular machinery underlies the specific functions of each hippocampal subfield.
  • Findings deepen the understanding of hippocampal neurobiology and function.