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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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Updated: Aug 25, 2025

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

Targeted MicroRNA Profiling in Gastric Cancer with Clinical Assessement.

H Pehlevan Özel¹, T Dinç¹, R S Tiryaki²

¹Department of General Surgery, Health Sciences University, Ankara City Hospital, Ankara, Turkey.

Balkan Journal of Medical Genetics : BJMG

|October 17, 2022

Summary

This summary is machine-generated.

This study identified six microRNAs (miRNAs) significantly underexpressed in gastric cancer tissues, suggesting their potential as diagnostic biomarkers for stomach cancer. These findings aid in developing new tools for gastric cancer detection.

Keywords:

Gastric adenocarcinoma tissues Gastric cancer Gastric cancer patients Metastatic lymph nodes microRNAs (miRNAs)

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Last Updated: Aug 25, 2025

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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

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Published on: September 18, 2020

Area of Science:

Oncology
Molecular Biology
Biomarker Discovery

Background:

Gastric cancer lacks stable and validated biomarkers.
MicroRNAs (miRNAs) show promise as potential biomarkers.
Identifying specific miRNAs in gastric adenocarcinoma is crucial.

Purpose of the Study:

To identify and validate gastric cancer-specific miRNAs.
To determine alterations in miRNA levels in gastric adenocarcinoma tissues.
To use paired normal and tumor samples for validation.

Main Methods:

Studied miRNA expression in 20 paired gastric adenocarcinoma and normal stomach tissues.
Utilized quantitative reverse transcription polymerase chain reaction (RT-qPCR) for eight miRNAs.
Analyzed miRNA expression profiling in 40 tissue samples.

Main Results:

Six miRNAs (miR-375-3p, hsamiR-129-5p, miR-196a-5p, miR-376c-3p, miR-34c-5p, miR-767-5p) were significantly underexpressed in tumors.
miR-129-3p and miR34c-5p levels correlated with lymph node metastasis.
miR-376c-3p levels were negatively associated with smoking.

Conclusions:

Identified specific miRNAs as potential biomarkers for gastric cancer.
Alterations in miRNA expression can aid in developing diagnostic tools.
Lower miRNA expression variability in cancerous tissues compared to normal tissues was observed.