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Self-coordinated nanozyme on Cu3BiS3 nanorods for high-performance aptasensing
Yanru Chen1, Lingling Zhao1, Xiuming Wu1
1Key Laboratory of Synthetic and Biological Colloids (Ministry of Education), School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China.
Mikrochimica Acta
|October 17, 2022
Summary
Researchers developed a novel nanozyme using ferrocyanides on Cu3BiS3 nanorods for sensitive biosensing. This engineered nanozyme enables label-free detection of chloramphenicol with high sensitivity and a low limit of detection.
Area of Science:
- Nanomaterials Science
- Catalysis
- Biosensing
Background:
- Nanozymes offer promising catalytic and sensing properties.
- Developing cost-effective and high-performance nanozymes is crucial for advanced bioassays.
- Controlling nanozyme formation is key to creating versatile biosensing platforms.
Purpose of the Study:
- To develop a novel nanozyme through self-coordination of ferrocyanides onto Cu3BiS3 nanorods.
- To engineer a tunable nanozyme for a homogeneous, label-free aptasensing platform.
- To demonstrate the utility of the engineered nanozyme in detecting chloramphenicol.
Main Methods:
- Fabrication of Cu3BiS3 (CBS) nanorods.
- In situ self-coordination of ferrocyanides ([Fe(CN)6]4-) onto CBS nanorods.
- Surface modification with deoxyribonucleoside 5'-monophosphates (dNMP) to tune nanozyme formation.
- Colorimetric detection of chloramphenicol (CAP) using 3,3',5,5'-tetramethylbenzidine (TMB) as a substrate.
Main Results:
- High-performance nanozymes were successfully synthesized with high catalytic activity and stability.
- The engineered nanozyme enabled tunable formation, facilitating a homogeneous, label-free aptasensing platform.
- The aptasensor demonstrated high sensitivity for chloramphenicol detection, with a limit of detection of 0.033 pM.
Conclusions:
- The novel self-coordinated nanozyme strategy provides a cost-effective and scalable method for producing high-performance nanozymes.
- The engineered nanozyme serves as a universal signal transduction scaffold for versatile bioassay applications.
- This approach enables the development of sensitive and convenient label-free biosensing platforms.
