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Updated: Aug 25, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Genetically encodable tagging and sensing systems for fluorescent RNA imaging

Zhimei Huang1, Xiaoyan Guo1, Xianbo Ma1

  • 1State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.

Biosensors & Bioelectronics
|October 17, 2022
PubMed
Summary

Genetically encodable tagging and sensing (GETS) systems enable live cell imaging of RNA dynamics. These advanced tools, including CRISPR-Cas9/13, offer powerful methods for RNA labeling, tracking, and sensing in living systems.

Keywords:
CRISPR-dCas9/13Fluorescent proteinsFluorogenic RNA aptamersGenetically encodable systemsRNA imaging

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