Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Controlled-Potential Coulometry: Electrolytic Methods01:17

Controlled-Potential Coulometry: Electrolytic Methods

246
Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential...
246
Bioremediation00:46

Bioremediation

19.8K
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.
19.8K
Standard Electrode Potentials03:02

Standard Electrode Potentials

44.8K
On comparing the reactivity of silver and lead, it is observed that the two ionic species, Ag+ (aq) and Pb2+ (aq), show a difference in their redox reactivity towards copper: the silver ion undergoes spontaneous reduction, while the lead ion does not. This relative redox activity can be easily quantified in electrochemical cells by a property called cell potential. This property is commonly known as cell voltage in electrochemistry, and it is a measure of the energy which accompanies the charge...
44.8K
Metabolism of Chemolithotrophs01:15

Metabolism of Chemolithotrophs

110
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
110

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plant-associated microbial enrichment is linked to trace emerging contaminant removal in full-scale constructed wetland.

Bioresource technology·2026
Same author

Patterns and mechanisms of cross-media antimicrobial resistance development in a typical reclaimed water-receiving urban river.

Water research·2026
Same author

From fixed to condition-dependent emission factors: probabilistic tabular learning for wastewater N<sub>2</sub>O inventories.

Water research·2026
Same author

Temporal sulfur redox reprogramming enables control-oriented intensification of sulfur-based autotrophic denitrification.

Water research·2026
Same author

Assessing global flood-induced occurrence and transmission risks of biological contaminants.

Water research·2026
Same author

Making waves: Model transfer as a key pathway to improving the generalization of machine learning in wastewater treatment engineering.

Water research·2026

Related Experiment Video

Updated: Aug 25, 2025

Characterizing Electron Transport through Living Biofilms
08:52

Characterizing Electron Transport through Living Biofilms

Published on: June 1, 2018

8.5K

Cathode potential regulates the microbiome assembly and function in electrostimulated bio- dechlorination system.

Di Cao1, Zhi-Ling Li1, Ke Shi1

  • 1State Key Laboratory of Urban Water Resources and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.

Journal of Hazardous Materials
|October 17, 2022
PubMed
Summary

Cathode potential significantly influences microbial communities in electro-stimulated dechlorination. Lower potentials enhance dechlorination rates and promote cooperative microbial interactions for efficient bio-dechlorination.

Keywords:
2,4,6-trichlorophenolElectrostimulationMicrobial community structure and assemblyMicrobial molecular networkReductive dichlorination

More Related Videos

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization
11:58

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization

Published on: December 29, 2013

13.5K
Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site
05:29

Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site

Published on: July 24, 2018

7.7K

Related Experiment Videos

Last Updated: Aug 25, 2025

Characterizing Electron Transport through Living Biofilms
08:52

Characterizing Electron Transport through Living Biofilms

Published on: June 1, 2018

8.5K
Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization
11:58

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization

Published on: December 29, 2013

13.5K
Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site
05:29

Self-standing Electrochemical Set-up to Enrich Anode-respiring Bacteria On-site

Published on: July 24, 2018

7.7K

Area of Science:

  • Environmental microbiology
  • Electrochemistry
  • Bioremediation

Background:

  • Microbial reductive dechlorination is crucial for degrading chlorinated pollutants.
  • Understanding microbiome assembly and function in electro-stimulated systems is key.
  • The role of cathode potential in driving these processes is not well understood.

Purpose of the Study:

  • Investigate how cathode potential affects microbiome structure, interactions, and function.
  • Analyze the assembly of core microbiomes in a 2,4,6-trichlorophenol bio-dechlorination system.
  • Determine the optimal cathode potential for efficient bio-dechlorination.

Main Methods:

  • Electrically stimulated microbial reductive dechlorination experiments.
  • Analysis of microbiome structure using taxonomic and functional profiling.
  • Investigation of microbial interactions and network analysis.
  • Measurement of dechlorination rates and metabolite analysis.

Main Results:

  • The highest dechlorination rate (24.30 μM/d) occurred at -0.36 V, producing phenol.
  • Lower or higher potentials reduced the dechlorination kinetic constant by 1.3-3.8 times.
  • Lower cathode potentials increased methane (CH4) generation, indicating hydrogenotrophic methanogenesis.
  • Microbiome structure shifted significantly at -0.36 V and -0.56 V, enriching dechlorinators, fermenters, and methanogens.
  • Simplified and strengthened cooperation among functional genera was observed at -0.36 V.

Conclusions:

  • Cathode potential is a major driver of core microbiome assembly in electro-stimulated dechlorination.
  • Low cathode potentials promote deterministic assembly of key microbial groups (dechlorinators, methanogens, electro-active genera).
  • Fermenting genera assembly appears more governed by stochastic processes.
  • Optimized cathode potential can enhance microbial cooperation for efficient bioremediation.