Small peptide glutathione-induced bioflocculation for enhancing the food application potential of Chlorella...

Existing flocculants are used to enhance the harvesting efficiency of microalgae; however, harvesting biomass containing residues is unsuitable for food applications. In this study, a small peptide-induced bioflocculation technique was developed for harvesting microalgae, and the biomass was free of impurities. After seven days of cultivation with glutathione, 72 % flocculation efficiency of Chlorella pyrenoidosa was achieved after settling for 1 h. The nutrient composition of flocs depicted a higher protein (68.94 mg/L) and lipid (48.97 mg/L) content than those of the control (65.91 and 41.44 mg/L). The amino acid profiles of flocs showed the presence of more essential amino acids than in untreated cells. More omega polyunsaturated fatty acids, such as ω-3 and ω-9, accumulate in flocs. Extracellular polymeric substances, which induced bioflocculation, appeared markedly in flocs (150.02 mg/L) compared to the control (32.30 mg/L). This study provides novel insights into the residue-free algal harvesting method and obtained nutrition-enriched biomass.