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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

870
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
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Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

1.0K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
1.0K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.7K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Mass Spectrometers01:16

Mass Spectrometers

5.9K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
5.9K
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

884
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Updated: Aug 25, 2025

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

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Emerging Computational Methods in Mass Spectrometry Imaging.

Hang Hu1, Julia Laskin1

  • 1Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN, 47907, USA.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|October 17, 2022
PubMed
Summary
This summary is machine-generated.

Computational methods, particularly artificial intelligence, are crucial for analyzing the vast data generated by mass spectrometry imaging (MSI). These approaches enhance MSI capabilities, improving data analysis and experimental throughput for molecular mapping.

Keywords:
artificial intelligencecomputational methodsdata miningdata-driven experimentsmass spectrometry imaging

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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
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Area of Science:

  • Analytical Chemistry
  • Computational Biology
  • Biotechnology

Background:

  • Mass spectrometry imaging (MSI) offers high-sensitivity molecular mapping in biological samples.
  • Advanced MSI generates large datasets, requiring sophisticated computational tools for analysis.
  • Computation-driven experiments are emerging to enhance MSI capabilities without hardware changes.

Purpose of the Study:

  • To critically review computational methods and resources for MSI data analysis and interpretation.
  • To summarize computational approaches for improving MSI throughput and molecular coverage.
  • To highlight the role of artificial intelligence in advancing MSI.

Main Methods:

  • Review of existing literature on computational tools for MSI.
  • Focus on artificial intelligence (AI) methods applied to MSI data.
  • Discussion of computation-driven MSI experimental strategies.

Main Results:

  • A comprehensive summary of computational methods for MSI data analysis.
  • Identification of AI as a key driver for MSI advancements.
  • Outlook on transformative computational methods shaping the future of MSI.

Conclusions:

  • Computational tools, especially AI, are essential for unlocking the full potential of MSI.
  • AI promises a paradigm shift in MSI capabilities, enhancing data interpretation and experimental design.
  • Future MSI applications will be heavily influenced by advanced computational strategies.