Related Experiment Video

Updated: Aug 25, 2025

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells
10:21

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Published on: January 5, 2018

13.2K

CRISPR/Cas9: A revolutionary genome editing tool for human cancers treatment

Fatima Akram1, Ikram Ul Haq1,2, Sania Sahreen1

  • 1Institute of Industrial Biotechnology, 66878Government College University, Lahore, Pakistan.

Technology in Cancer Research & Treatment
|October 18, 2022
PubMed

Insights

Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology offers promising cancer diagnostic and treatment strategies, including immunotherapy and disease modeling. Further research is needed to overcome challenges like off-target effects for full clinical application.

Area of Science:

  • Oncology
  • Genetics
  • Biotechnology

Background:

  • Cancer is a leading global cause of death, driven by genetic and epigenetic mutations.
  • Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 is a revolutionary gene-editing tool with significant potential in oncology.
  • CRISPR/Cas9 has been instrumental in developing preclinical cancer models, such as those for medulloblastoma and glioblastoma.

Purpose of the Study:

  • To review the role and applications of CRISPR/Cas9 technology in cancer diagnosis and treatment.
  • To highlight the use of CRISPR/Cas9 in developing cancer models and its application in immunotherapy.
  • To discuss the existing challenges and potential solutions for the clinical implementation of CRISPR/Cas9 in cancer care.

Main Methods:

  • Literature review of CRISPR/Cas9 applications in cancer research and treatment.
  • Analysis of CRISPR/Cas9's diagnostic capabilities for detecting cancer-related genes.
  • Examination of CRISPR/Cas9-mediated cancer immunotherapy and its integration into treatment protocols.

Main Results:

  • CRISPR/Cas9 facilitates rapid and efficient detection of genes implicated in cancer development, proliferation, metastasis, and drug resistance.
  • CRISPR/Cas9 technology is effectively used in developing various cancer models and as a component of cancer immunotherapy.
  • Significant challenges remain, including off-target effects, editing efficiency, delivery methods, and homology-directed repair (HDR) efficiency.

Conclusions:

  • CRISPR/Cas9 technology represents a paradigm shift in cancer diagnosis and treatment, offering novel therapeutic avenues.
  • Addressing the technical and biological challenges is crucial for the successful clinical translation of CRISPR/Cas9-based cancer therapies.
  • Continued research and development are essential to fully harness the potential of CRISPR/Cas9 for comprehensive cancer management.
Keywords:
CRISPR/Cas9cancerdiagnosisgene editingimmunotherapy

More Related Videos

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing09:03

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

4.2K
Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells12:04

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

3.7K

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
145
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.7K
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.9K