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Hemagglutinating activity in phytopathogenic bacteria surface compounds.

M T Serra, M C Castresana, G Tejerina

    Journal of Basic Microbiology
    |January 1, 1987
    PubMed
    Summary

    NaCl extracts from plant pathogenic bacteria revealed hemagglutinating activity, particularly from Erwinia carotovora subsp. atroseptica. This activity, sensitive to carbohydrates, suggests a role for bacterial hemagglutinins in plant-parasite interactions.

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    Area of Science:

    • Microbiology
    • Plant Pathology
    • Biochemistry

    Background:

    • Plant pathogenic bacteria possess extracellular components that mediate interactions with host plants.
    • Hemagglutinins, molecules that cause red blood cells to clump, are found in various biological systems and can play roles in adhesion and recognition.

    Purpose of the Study:

    • To investigate the presence and characteristics of hemagglutinating activity in extracellular components of several plant pathogenic bacteria.
    • To explore the potential role of these bacterial hemagglutinins in the plant-parasite relationship.

    Main Methods:

    • Extracellular components were extracted from six species of plant pathogenic bacteria using NaCl, sucrose, or Na2EDTA.
    • Bacterial extracts were assayed for hemagglutinating activity against sheep, rabbit, and chicken red blood cells (RBCs).

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  • The effect of different carbohydrates on hemagglutinating activity was assessed, and polysaccharide/protein ratios were determined.

    • Main Results:

    • Only NaCl extracts exhibited hemagglutinating activity, primarily agglutinating sheep and rabbit erythrocytes.
    • Erwinia carotovora subsp. atroseptica extracts showed the highest agglutination titer on rabbit RBCs and had the lowest polysaccharide/protein ratio.
    • Hemagglutinating activity varied in sensitivity to carbohydrate inhibition among bacterial species, with E. carotovora subsp. atroseptica being most sensitive and E. carotovora subsp. carotovora least sensitive.

    Conclusions:

    • Hemagglutinins are present in the extracellular components of plant pathogenic bacteria, particularly those extracted with NaCl.
    • The observed hemagglutinating activity, especially in E. carotovora subsp. atroseptica, may be linked to bacterial surface polysaccharides and proteins.
    • Bacterial hemagglutinins might share similarities with host plant hemagglutinins and could be involved in the plant-pathogen interaction.