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ATF7-dependent epigenetic changes induced by high temperature during early porcine embryonic development
Ming-Hong Sun1, Wen-Jie Jiang1, Xiao-Han Li1
1Department of Animal Science, Chungbuk National University, Cheongju, South Korea.
Cell Proliferation
|October 18, 2022
Summary
Activating transcription factor 7 (ATF7) is crucial for early pig embryo development. Stress impacts ATF7, affecting key epigenetic markers and embryo quality, highlighting its role in development.
Area of Science:
- Epigenetics
- Developmental Biology
- Molecular Biology
Background:
- Activating transcription factor 7 (ATF7) is a transcription factor involved in heterochromatin formation.
- ATF7 is phosphorylated by stress-activated kinase P38 in response to stressors.
- The function of ATF7 in early porcine embryonic development is not well understood.
Purpose of the Study:
- To investigate the role of ATF7 in early porcine embryonic development.
- To understand how stress affects ATF7 and its impact on embryonic development.
Main Methods:
- Studied ATF7 localization and expression during porcine embryonic development.
- Utilized ATF7 knockdown and P38 activity inhibition.
- Assessed blastocyst rates, cell numbers, and expression of HP1 and H3K9me2.
Main Results:
- ATF7 accumulates in the nucleus and localizes to pericentric heterochromatin, co-localizing with HP1.
- ATF7 knockdown reduces blastocyst rate and cell number, downregulating HP1 and H3K9me2.
- P38 inhibition alleviates ATF7 depletion effects; high temperatures increase pP38, reduce embryo quality, and phosphorylate ATF7.
Conclusions:
- Stress-induced ATF7-dependent epigenetic changes are vital for early porcine embryonic development.
- ATF7 plays a significant role in regulating heterochromatin and embryonic development under stress conditions.
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