Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimizing scheduling in dual-pulse nucleoside labeling experiments for cell-cycle analysis.

Biophysical journal·2026
Same author

Practical AI-based cell extraction and spatial statistics for large 3D bone marrow tissue images.

Cell reports methods·2026
Same author

Author Correction: Macrophages excite muscle spindles with glutamate to bolster locomotion.

Nature·2025
Same author

Balancing hematopoietic stem cell self-renewal and differentiation activities throughout ontogeny and aging.

Experimental hematology·2025
Same author

Tumor-Infiltrating Clonal Hematopoiesis.

The New England journal of medicine·2025
Same author

Differential Response and Recovery Dynamics of HSPC Populations Following <i>Plasmodium chabaudi</i> Infection.

International journal of molecular sciences·2025

Related Experiment Video

Updated: Aug 25, 2025

Intravital Longitudinal Imaging of Vascular Dynamics in the Calvarial Bone Marrow
11:05

Intravital Longitudinal Imaging of Vascular Dynamics in the Calvarial Bone Marrow

Published on: April 11, 2025

442

Intravital Microscopy for Hematopoietic Studies.

Myriam L R Haltalli1,2,3, Cristina Lo Celso4,5

  • 1Imperial College London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|October 18, 2022
PubMed
Summary

Researchers visualized transplanted hematopoietic stem cells (HSCs) within the mouse bone marrow niche using advanced intravital microscopy. This technique allows real-time study of HSC interactions within their microenvironment.

Keywords:
3-dimensional image analysisBone marrowBone marrow microenvironmentConfocal and Multiphoton microscopyHematopoietic stem cellIntravital imagingNicheTransplantation

More Related Videos

Combining Intravital Fluorescent Microscopy IVFM with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
11:06

Combining Intravital Fluorescent Microscopy IVFM with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

Published on: March 21, 2017

8.0K
Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow
11:36

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

Published on: January 14, 2012

18.3K

Related Experiment Videos

Last Updated: Aug 25, 2025

Intravital Longitudinal Imaging of Vascular Dynamics in the Calvarial Bone Marrow
11:05

Intravital Longitudinal Imaging of Vascular Dynamics in the Calvarial Bone Marrow

Published on: April 11, 2025

442
Combining Intravital Fluorescent Microscopy IVFM with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches
11:06

Combining Intravital Fluorescent Microscopy IVFM with Genetic Models to Study Engraftment Dynamics of Hematopoietic Cells to Bone Marrow Niches

Published on: March 21, 2017

8.0K
Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow
11:36

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

Published on: January 14, 2012

18.3K

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Microscopy

Background:

  • The bone marrow (BM) microenvironment, or HSC niche, supports hematopoietic stem cells (HSCs) and their progeny.
  • HSCs require niche signaling for long-term function and survival.
  • Understanding HSC-niche interactions is crucial for hematopoiesis research.

Purpose of the Study:

  • To describe a protocol for visualizing transplanted HSCs in the mouse calvarium.
  • To utilize advanced imaging techniques for real-time in vivo study of HSCs.
  • To investigate HSC interactions within their specific microenvironment.

Main Methods:

  • Development and application of intravital microscopy (IVM) techniques.
  • Combined confocal and two-photon microscopy for high-resolution imaging.
  • Visualization of transplanted HSCs in the mouse calvarium model.

Main Results:

  • Successful visualization of transplanted HSCs in vivo.
  • Demonstration of real-time imaging of HSCs within the bone marrow niche.
  • Establishment of a protocol for studying HSC-environment interactions.

Conclusions:

  • Intravital microscopy provides a powerful tool to study HSCs in their native microenvironment.
  • This technique facilitates the investigation of HSC behavior under various conditions.
  • Further research can explore specific niche requirements for distinct hematopoietic lineages.