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Traveling tunable laser projector for UV-blue disinfection dose determinations

Thomas Larason, Steven Grantham, Clarence Zarobila

    Applied Optics
    |October 18, 2022
    PubMed

    Insights

    Researchers studied SARS-CoV-2 inactivation using UV and blue light. A pulsed laser system effectively inactivated the virus, demonstrating its value for future research on light-based pathogen control.

    Area of Science:

    • Photochemistry
    • Microbiology
    • Biophysics

    Background:

    • The COVID-19 pandemic highlighted the need for effective SARS-CoV-2 inactivation methods.
    • Ultraviolet (UV) and blue light are potential tools for pathogen control.
    • Previous research demonstrated UV laser irradiation's efficacy in water pathogen inactivation.

    Purpose of the Study:

    • To investigate the inactivation of SARS-CoV-2 using a novel pulsed laser and projection system.
    • To determine the effective doses of UV and blue light across a specific wavelength range for SARS-CoV-2 inactivation.
    • To build upon existing NIST research in UV laser-based pathogen inactivation.

    Main Methods:

    • Development and implementation of a 1 kHz pulsed laser and projection system.
    • Exposure of SARS-CoV-2 to light across wavelengths from 222 to 488 nm.
    • Operation and performance evaluation of the system within a Biosafety Level 3 (BSL-3) laboratory.

    Main Results:

    • The tunable laser projection system was successfully designed and operated in a BSL-3 environment.
    • The system enabled the study of SARS-CoV-2 inactivation doses across a broad spectrum of light.
    • SARS-CoV-2 inactivation results, detailed in a separate publication, confirmed the system's effectiveness.

    Conclusions:

    • A tunable laser projection system is a valuable tool for researching light-based SARS-CoV-2 inactivation.
    • This technology facilitates precise dose-response studies for various light wavelengths.
    • The findings support the development of advanced UV and blue light disinfection strategies.

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