Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

State-dependent energy conversion produces degenerate dissipation in active actomyosin networks.

bioRxiv : the preprint server for biology·2026

Same author

Mechanical movements generated by movable lipids break endosomal barriers for enhanced mRNA therapeutics.

Science advances·2026

Same author

A novel translation initiation codon single nucleotide variant of the SLC14A1 (c.2T>G) from a Chinese blood donor with Jk(a-b-) phenotype.

Transfusion·2026

Same author

A pico-calorimeter for cellular metabolism and antimicrobial susceptibility testing.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Soliton eigenvalue control by interaction of circularly polarized lights in a nonlinear fiber.

Optics express·2026

Same author

Synergistic microscopic platform for probing molecular interaction on mitochondria-lysosome contact membrane.

Biomedical optics express·2026

Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026

Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026

Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026

Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026

Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026

Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Aug 25, 2025

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Published on: May 20, 2014

Phase image correlation spectroscopy for detecting microfluidic dynamics.

Lan Yu, Yu Wang, Yang Wang

|October 18, 2022

Summary

This summary is machine-generated.

Phase Image Correlation Spectroscopy (PICS) quantifies unlabeled particle properties in microfluidics. This minimally invasive technique offers a simpler, more efficient alternative to traditional methods for analyzing particle concentration, size, and flow velocity.

More Related Videos

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

Published on: August 27, 2013

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

Related Experiment Videos

Last Updated: Aug 25, 2025

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Published on: May 20, 2014

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

Published on: August 27, 2013

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Published on: December 17, 2015

Area of Science:

Microfluidics
Biomedical Engineering
Particle Imaging

Background:

Quantifying physical properties and dynamics of flowing particles is crucial for microfluidic applications.
Conventional methods often rely on fluorescent labeling, which can be invasive and complex.

Purpose of the Study:

To introduce Phase Image Correlation Spectroscopy (PICS) as a novel, versatile tool for analyzing unlabeled flowing particles.
To demonstrate PICS's ability to quantify particle concentration, hydro-diameter, and flow velocity in microfluidic devices.

Main Methods:

PICS utilizes the intrinsic phase of particles to generate contrast, eliminating the need for fluorescent labeling.
Correlating pixel data from phase images of flowing particles within a microfluidic device.

Main Results:

Successfully quantified concentration, hydro-diameter, and flow velocity of polymethylmethacrylate (PMMA) microspheres and yeast.
Demonstrated PICS as a minimally invasive, simple, and efficient alternative to conventional techniques.

Conclusions:

PICS is a feasible and effective method for analyzing unlabeled flowing particles in microfluidics.
PICS has the potential to become an essential inspection tool in biomedicine and industry.