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Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

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Dissolved oxygen sensor based on the fluorescence quenching method with optimal modulation frequency.

Zhixuan Er, Ping Gong, Jian Zhou

|October 18, 2022

Summary

This summary is machine-generated.

A new dissolved oxygen (DO) sensor uses fluorescence quenching for accurate measurements. Optimized modulation frequency enhances sensor performance, achieving high accuracy for industrial and biomedical applications.

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Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

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Area of Science:

Analytical Chemistry
Sensor Technology
Biomedical Engineering

Background:

Accurate dissolved oxygen (DO) measurement is crucial for industrial processes and biomedical applications.
Existing DO measurement methods may have limitations in accuracy or applicability.
Fluorescence-based sensing offers a promising alternative for DO detection.

Purpose of the Study:

To develop and characterize a novel dissolved oxygen sensor utilizing the fluorescence quenching method.
To investigate the influence of modulation frequency on sensor performance.
To establish an accurate fitting model considering nonlinear effects of fluorescent materials.

Main Methods:

Fabrication of a dissolved oxygen sensor based on fluorescence quenching.
Utilizing fluorescence lifetime detection via phase difference analysis between excitation and emission signals.
Incorporating nonlinear effects of the fluorescent material into the fitting model.
Evaluating system performance across various modulation frequencies of the excitation light.

Main Results:

The developed sensor demonstrates a high degree of fit (R² = 0.9981) with an optimized modulation frequency.
A small relative error of 0.79% was achieved, indicating high measurement accuracy.
Modulation frequency was found to significantly impact sensor sensitivity and phase resolution.
The nonlinear effects of the fluorescent material were successfully integrated into the fitting model.

Conclusions:

The developed fluorescence quenching-based DO sensor exhibits excellent performance and accuracy.
Optimization of modulation frequency is key to maximizing sensor sensitivity and resolution.
The sensor is suitable for critical dissolved oxygen measurements in diverse industrial and biomedical fields.