64-channel label-free fluorescence detection and single-particle counting device

Area of Science:

• Biophotonics
• Cellular Biology
• Analytical Chemistry

Background:

• Cell-level fluorescence detection often requires labeling, complicating sample prep and increasing cytotoxicity.
• Label-free detection is crucial for in vivo research and minimizing biological interference.

Purpose of the Study:

• To establish and validate a 64-channel detection system for label-free, cell-level laser-induced fluorescence (LIF) analysis.
• To enable sensitive detection of feeble emissions for improved biological sample analysis.

Main Methods:

• Development of a 64-channel detection system incorporating two linear photodetector devices.
• System validation using unlabeled human hepatocytes (L-02) and other cell-level biologic samples.
• Application of the system for particle counting using a quartz microfluidic chip.

Main Results:

• The system achieves label-free detection of feeble emissions at the cellular level.
• The detection system covers a wavelength range of 366-680 nm with an average spectral resolution of 4.9 nm.
• Successful validation in detecting unlabeled human hepatocytes and performing particle counting.

Conclusions:

• The 64-channel LIF system offers a sensitive, label-free approach for cellular analysis.
• This technology advances in vivo research by simplifying sample preparation and reducing cytotoxicity.
• The system's spectral-based counting method provides an alternative to traditional irradiance intensity-based devices.