Platelet function testing using the Multiplate analyzer after administration of aspirin in Aachen minipigs

Area of Science:

• Comparative physiology
• Pharmacology
• Hematology

Background:

• Platelet function knowledge in pigs is vital for translating animal research to human medicine.
• Understanding antiplatelet therapy effectiveness in preclinical models is essential.

Purpose of the Study:

• Characterize baseline platelet function in Aachen minipigs using the Multiplate analyzer.
• Compare baseline platelet function between Aachen and Göttingen minipigs.
• Assess immediate platelet inhibition following intravenous acetylsalicylic acid (ASA) administration in Aachen minipigs.

Main Methods:

• Baseline platelet function and hematological parameters were assessed in 9 Aachen minipigs.
• Historical data from 8 unmedicated Göttingen minipigs were used for comparison.
• Multiplate assays included ASPI, adenosine-diphosphate (ADP), and thrombin receptor activating peptide (TRAP) tests.
• Platelet inhibition was measured 1-5 minutes after intravenous ASA (500 mg) administration.

Main Results:

• Aachen minipigs exhibited higher variability in baseline platelet function (ASPI: 39 U) compared to Göttingen minipigs (ASPI: 70.5 U).
• Göttingen minipigs showed significantly higher ASPI values (p = 0.046).
• Intravenous ASA administration in Aachen minipigs led to significant and immediate platelet inhibition within 1 minute, sustained for 5 minutes (p≤0.038).

Conclusions:

• Aachen minipigs demonstrate considerable variability in arachidonic acid-mediated platelet aggregation.
• Intravenous ASA effectively and rapidly inhibits platelet function in Aachen minipigs.
• These findings support the use of Aachen minipigs for preclinical antiplatelet therapy studies.