Physical, mechanical, and biological characterization of robocasted carbon nanotube reinforced microwave sintered calcium phosphate scaffolds for bone tissue engineering

This study analyses the influence of the addition of Multi Walled Carbon Nanotubes (MWCNT) on the physical, mechanical, and biological behaviour of Calcium Phosphate (CP) bone scaffolds developed using the robocasting technique for bone regeneration. Three different mass percentages (0.5, 1, and 2 wt%) of MWCNT are added to the CP powder and a slurry is prepared using a CMC binder for printing the scaffolds. The scaffolds were printed in 2 infill ratios, 50 and 100%, and were sintered under an inert atmosphere in a microwave furnace which was then taken for various characterization studies. Physical characterisation studies revealed that the shrinkage rate of scaffolds is very low compared to other additive manufacturing techniques. The incorporation of 0.5 wt% of MWCNT produced the best results in mechanical characterization studies with a compressive strength of 10.38 MPa and 11.89 MPa for 50% and 100% infill ratios respectively. In Vitro Biocompatibility studies also proved that 0.5 wt% MWCNT samples are the most suitable for cell growth while the hemocompatibility tests showed that the samples are blood compatible. . The 100% infill samples fared better than the 50% samples in physical and mechanical properties. The results suggest that the MWCNT incorporated CP scaffolds can be used to treat critical size bone defects.