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Quantifying cell adhesion through forces generated by acoustic streaming.

Chikahiro Imashiro1, Jiyang Mei2, James Friend2

  • 1Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan; Department of Mechanical Engineering, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8522, Japan.

Ultrasonics Sonochemistry
|October 18, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel system to measure cell adhesion strength using acoustic streaming. This bioengineering tool quantifies cell detachment forces, crucial for understanding cell health and culture.

Keywords:
Acoustic streamingCell adhesionCell detachmentLab on a chipLamb wave

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Area of Science:

  • Bioengineering
  • Cell Biology
  • Acoustics

Background:

  • Cell adhesion strength is vital for cell health and culture.
  • Quantifying cell adhesion strength presents a significant challenge in bioengineering.
  • Existing methods for measuring cell adhesion are limited.

Purpose of the Study:

  • To develop and validate a system for quantitative measurement of cell adhesion strength.
  • To utilize acoustic streaming induced by Lamb waves for cell detachment.
  • To establish a fundamental tool for cell-based bioengineering applications.

Main Methods:

  • Cells were cultured on an ultrasound transducer.
  • Acoustic streaming was induced using varying Lamb wave intensities to detach cells.
  • Cell detachment was analyzed to determine cell adhesion force, using a median detachment rate of 50%.

Main Results:

  • The developed system accurately measured cell adhesion strength on the order of several nN.
  • Increased shear flow from acoustic streaming resulted in greater cell detachment.
  • Cell adhesion forces ranging from 1.2 to 13 nN were observed with varied preculture and incubation times.

Conclusions:

  • The developed system provides a reliable method for quantifying cell adhesion strength.
  • This technique offers a tunable approach to cell detachment and adhesion force measurement.
  • The system has potential as a fundamental tool in cell-based bioengineering research and applications.