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Updated: Aug 25, 2025
Microfluidic Chip Fabrication and Method to Detect Influenza
Published on: March 26, 2013
Clustered Regularly Interspaced short palindromic repeats-Based Microfluidic System in Infectious Diseases Diagnosis:
Yi Xie1,2, Huimin Li1,2, Fumin Chen1,2
1School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, P. R. China.
Abstract:
Mitigating the spread of global infectious diseases requires rapid and accurate diagnostic tools. Conventional diagnostic techniques for infectious diseases typically require sophisticated equipment and are time consuming. Emerging clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) detection systems have shown remarkable potential as next-generation diagnostic tools to achieve rapid, sensitive, specific, and field-deployable diagnoses of infectious diseases, based on state-of-the-art microfluidic platforms. Therefore, a review of recent advances in CRISPR-based microfluidic systems for infectious diseases diagnosis is urgently required. This review highlights the mechanisms of CRISPR/Cas biosensing and cutting-edge microfluidic devices including paper, digital, and integrated wearable platforms. Strategies to simplify sample pretreatment, improve diagnostic performance, and achieve integrated detection are discussed. Current challenges and future perspectives contributing to the development of more effective CRISPR-based microfluidic diagnostic systems are also proposed.
Insights
Rapid infectious disease diagnosis is crucial. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) systems integrated with microfluidics offer a promising solution for sensitive, field-deployable diagnostics.
Area of Science:
- Biotechnology
- Molecular Diagnostics
- Medical Devices
Background:
- Global infectious disease spread necessitates advanced diagnostic tools.
- Conventional methods are often slow and require specialized equipment.
- CRISPR/Cas systems offer a novel approach for rapid and accurate detection.
Purpose of the Study:
- To review recent advancements in CRISPR-based microfluidic systems for infectious disease diagnosis.
- To highlight the potential of these systems as next-generation diagnostic tools.
- To discuss strategies for improving diagnostic performance and integration.
Main Methods:
- Review of CRISPR/Cas biosensing mechanisms.
- Analysis of cutting-edge microfluidic platforms (paper, digital, wearable).
- Discussion of sample pretreatment simplification and integrated detection strategies.
Main Results:
- CRISPR/Cas systems demonstrate high sensitivity and specificity.
- Microfluidic integration enables rapid, field-deployable diagnostics.
- Various platforms show potential for diverse diagnostic applications.
Conclusions:
- CRISPR-based microfluidic systems represent a significant advancement in infectious disease diagnostics.
- Further development is needed to address current challenges and enhance clinical utility.
- These technologies hold promise for improved global health surveillance and response.
