Comparative study of effective antibiofilm activity of beneficial microbes-mediated zirconia nanoparticles

In the present study, beneficial microbes-mediated zirconia nanoparticles were prepared using endophytic bacteria isolated from the seed of Terminalia chebula which were evaluated on inhibition of bacterial adherence and promotion to exhibit antibiofilm properties. The structure and distribution of the zirconia nanoparticles were examined through SEM (Scanning Electron Microscopy), EDS (Energy-Dispersive X-Ray spectroscopy), and XRD (X-ray diffraction analysis), which reveal the distribution of the particles. The morphology of biogenic zirconia nanoparticles was monoclinic and cubic. The formation of zirconia particle was confirmed using UV spectrum and the functional groups were intensified in FTIR (Fourier-transform infrared spectroscopy). The antibiofilm activity of the synthesized nanoparticles was tested in oral pathogens that cause biofilm by membrane integrity and leads to periodontal associated disease. The results showed that the particles had a significant effect on biofilm removal caused by oral pathogens. For determined concentration, the cytotoxicity of the endophytic bacterial facilitated zirconia nanoparticle (Zr NPs) was examined in HGF (Human gingival fibroblast cell line).