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Related Experiment Video

Updated: Aug 25, 2025

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

In vivo visualization of nitrate dynamics using a genetically encoded fluorescent biosensor.

Yen-Ning Chen¹, Heather N Cartwright², Cheng-Hsun Ho¹

¹Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan.

Science Advances

|October 19, 2022

Summary

Researchers developed NitraMeter3.0, a novel fluorescent biosensor, to visualize nitrate (NO₃^-) distribution in plants. This tool allows real-time, high-resolution tracking of nitrate levels within plant cells, advancing plant science research.

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Last Updated: Aug 25, 2025

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

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Area of Science:

Plant Biology
Molecular Biology
Biochemistry

Background:

Nitrate (NO₃^-) is essential for plant growth and development.
Understanding nitrate uptake and distribution is crucial for plant physiology.
Current methods lack high-resolution cellular-level visualization of nitrate dynamics.

Purpose of the Study:

To develop a genetically encoded fluorescent biosensor for visualizing nitrate distribution in plants.
To enable quantitative, real-time, and high-resolution monitoring of nitrate at the cellular level.
To investigate nitrate spatiotemporal distribution in *Arabidopsis thaliana*.

Main Methods:

Development of a nuclear-localized genetically encoded fluorescent biosensor (NitraMeter3.0).
Application of the biosensor in *Arabidopsis thaliana* to track nitrate distribution.
Utilizing the biosensor to analyze disruptions in nitrate transport and assimilation mutants.

Main Results:

NitraMeter3.0 successfully visualized the spatiotemporal distribution of nitrate along the primary root axis.
The biosensor effectively monitored cellular nitrate concentrations in real-time.
Observed disruptions in nitrate distribution due to genetic mutations affecting transport and assimilation.

Conclusions:

NitraMeter3.0 provides a powerful tool for quantitative visualization of cellular nitrate dynamics.
The biosensor facilitates real-time monitoring of nitrate spatiotemporal changes throughout the plant life cycle.
This technology advances the study of nitrate uptake, transport, and assimilation in plants.