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Laticifer growth pattern is guided by cytoskeleton organization.
Maria Camila Medina1, Mariane S Sousa-Baena1, Marie-Anne Van Sluys1
1Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo, SP, Brazil.
Frontiers in Plant Science
|October 20, 2022
Summary
This study reveals that laticifers, plant secretory structures, exhibit diffuse growth. Microtubule orientation indicates this growth pattern in both nonanastomosing and anastomosing laticifers.
Area of Science:
- Plant Biology
- Cell Biology
- Developmental Biology
Background:
- Laticifers are plant secretory structures producing latex for defense.
- Their growth mode (intrusive vs. diffuse) and the role of cytoskeleton are poorly understood.
Purpose of the Study:
- To investigate laticifer growth patterns using developmental anatomy and microtubule immunostaining.
- To determine if laticifer growth is intrusive or diffuse.
Main Methods:
- Standard anatomical methods for laticifer development.
- Microtubule immunolocalization of α-tubulin in three plant species.
- Analysis of microtubule orientation in different laticifer types.
Main Results:
- Microtubules were perpendicularly aligned to the cell growth axis in laticifers and other plant cells.
- This orientation indicates diffuse growth in nonanastomosing and anastomosing laticifers.
- Laticifer growth was confirmed as diffuse, regardless of branching or anastomosis.
Conclusions:
- Laticifer growth is predominantly diffuse, challenging previous assumptions.
- Cytoskeleton, specifically microtubules, plays a role in establishing laticifer growth patterns.
- This study advances the understanding of secretory structure development and function.
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