Expression pattern and clinical value of Key RNA methylation modification regulators in ischemic stroke

Area of Science:

• Epigenetics
• Neuroscience
• Bioinformatics

Background:

• Ischemic stroke (IS) is a leading cause of death and disability globally.
• Effective diagnostic and treatment strategies for IS remain limited.
• The role of RNA methylation in IS pathogenesis is largely unexplored.

Purpose of the Study:

• To investigate RNA methylation patterns and immune infiltration in IS using bioinformatics.
• To identify novel diagnostic biomarkers and therapeutic targets for IS.

Main Methods:

• Downloaded and analyzed gene expression profiles from the Gene Expression Omnibus database.
• Utilized subtype classification based on RNA methylation regulators, differential gene expression analysis, and weighted gene co-expression network analysis.
• Constructed a clinical diagnostic model and validated characteristic genes using ROC curve and qPCR.

Main Results:

• Identified four significantly upregulated IS characteristic genes: GFAP, GPNMB, FKBP9, and CHMP5.
• Established a validated clinical diagnostic model for IS.
• Revealed correlations between characteristic genes and immune cell infiltration.
• Predicted chloroquine as a potential therapeutic drug targeting GPNMB.

Conclusions:

• RNA methylation regulators play a significant role in IS.
• GFAP, GPNMB, FKBP9, and CHMP5 are promising diagnostic biomarkers for IS.
• GPNMB and its regulatory network present novel therapeutic avenues for IS.