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Design Example: Capacitance Multiplier Circuit

Design Example: Capacitance Multiplier Circuit

In integrated circuit technology, a capacitance multiplier is often utilized to produce a larger capacitance value when a small physical capacitance falls short. This is achieved by a circuit that multiplies capacitance values by a factor of up to 1000, such that a 10-pF capacitor can replicate the performance of a 100-nF capacitor.
The circuit illustrated in Figure 1 below incorporates two op-amps, with the first operating as a voltage follower and the second acting as an inverting amplifier.

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Calculating subtransient fault currents for three-phase faults in an N-bus power system involves using the positive-sequence network. When a three-phase short circuit occurs at a specific bus, the analysis uses the superposition method to evaluate two separate circuits.
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Related Experiment Video

Updated: Aug 24, 2025

Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry

Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry

Published on: July 26, 2019

TMT29 plex: Higher order multiplexing with inherent interference correction.

¹Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.

|October 20, 2022

Summary

This summary is machine-generated.

This study introduces a TMT29-plex workflow combining TMT11-plex and TMTpro18-plex strategies to overcome ratio compression in mass spectrometry. This approach enhances protein quantification accuracy and assay throughput for complex samples.

Keywords:

29-plex TMT interference ratio compression ratio restoration

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Area of Science:

Proteomics
Analytical Chemistry
Biotechnology

Background:

Tandem Mass Tag (TMT) technology is crucial for multiplexed protein abundance profiling using mass spectrometry.
Increasing TMT channel numbers (from 2 to 18) enhances assay throughput.
Ratio compression in TMT experiments confounds reporter tag intensity measurements, especially in complex samples.

Purpose of the Study:

To present a novel TMT29-plex workflow to address ratio compression in TMT experiments.
To improve quantitative accuracy and throughput in mass spectrometry-based proteomics.
To leverage inherent features of TMT11-plex and TMTpro18-plex for enhanced performance.

Main Methods:

Development of a TMT29-plex workflow by combining TMT11-plex and TMTpro18-plex labeling.
Application of the workflow to complex biological samples.
Analysis of reporter tag intensities to assess ratio compression and quantitative accuracy.

Main Results:

The TMT29-plex workflow effectively mitigates the ratio compression problem.
Increased throughput and improved quantitative accuracy were achieved compared to existing methods.
The workflow demonstrated potential for advanced applications in protein profiling.

Conclusions:

The presented TMT29-plex workflow offers a robust solution for ratio compression in TMT-based proteomics.
This advancement significantly enhances the capability of mass spectrometry for high-throughput protein quantification.
The strategy holds promise for broader applications in biological and clinical research.