A FRET-based ratiometric fluorescent probe for Hg²⁺ detection in aqueous solution and bioimaging in multiple samples

Mercury ion, as a metal cation with great toxic effect, is widely present in various production and living environments. It seriously threatens human health and environmental safety. It is of great significance to develop convenient and effective methods for mercury ion detection. Here, we designed and synthesized a new ratiometric fluorescent probe (namely APS-NA) for the detection of mercury ions in the environment and multiple biological samples. The probe is constructed by covalently connecting two fluorophores with lipolic acid to achieve fluorescence resonance energy transfer (FRET). In the molecular structure of APS-NA, acridone is used as an energy donor, 1,8-naphthalimide is used as an energy acceptor, and a dithioacetal group is used as the reaction site for Hg²⁺. The intact APS-NA mainly shows the green fluorescence from the acceptor moiety 1,8-naphthalimide; the presence of Hg²⁺ ions would break the dithioacetal linkage between acridone and 1,8-naphthalimide; the defunctionalization of FRET would lead to bright blue fluorescence emission of acridone; thus ratiometric fluorescent detection of Hg²⁺ can be achieved by this recognition process. The probe not only has a large Stokes shift (Δλ = 110 nm), but also has high selectivity, high sensitivity (low detection limit 30 nM) and naked eye visualization. In addition, we have successfully used this probe for the detection Hg²⁺ of actual samples and imaging of a variety of organisms. These results indicate that the probe has broad application prospects.