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Updated: Aug 13, 2026

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
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Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans

Published on: February 16, 2017

Interaction of multiplication stimulating activity/rat insulin-like growth factor II with skeletal muscle satellite

M V Dodson, R E Allen

    Mechanisms of Ageing and Development
    |July 1, 1987
    PubMed

    Insights

    Young rat muscle cells show faster growth due to more insulin-like growth factor II (IGF-II) receptors. This finding is crucial for understanding muscle regeneration and aging.

    Area of Science:

    • Muscle biology
    • Cellular aging
    • Endocrinology

    Background:

    • Satellite cells are crucial for muscle repair and regeneration.
    • Aging affects satellite cell function and muscle regenerative capacity.
    • Insulin-like growth factor II (IGF-II) plays a role in cell growth and proliferation.

    Purpose of the Study:

    • To investigate the age-related differences in satellite cell growth and response to IGF-II.
    • To examine the characteristics of IGF-II binding sites on satellite cells from rats of different ages.

    Main Methods:

    • Isolation and in vitro culture of skeletal muscle satellite cells from 3-, 12-, and 24-month-old Fischer 344 rats.
    • Assessment of cell growth in response to multiplication stimulating activity/rat insulin-like growth factor II (MSA).
    • Hormone-binding assays using [125I]MSA to determine receptor number and affinity.

    Main Results:

    • Satellite cells from younger rats (3 months) exhibited a shorter lag phase in growth compared to older rats (12 and 24 months).
    • Dose-response curves for MSA showed no significant age-related differences in the required concentrations or magnitude of response.
    • Younger rat cells displayed higher numbers of MSA-binding sites with lower affinities, while older rat cells showed the opposite.

    Conclusions:

    • Age-related differences in satellite cell proliferation are linked to variations in IGF-II receptor characteristics.
    • Younger rats possess more numerous, lower-affinity IGF-II binding sites, potentially due to increased IGF type I receptors.
    • These findings suggest a mechanism for altered muscle regeneration capacity with aging.

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