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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Updated: Aug 24, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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Detection technologies for RNA modifications.

Yan Zhang1, Liang Lu1,2, Xiaoyu Li3

  • 1Department of Biochemistry and Department of Gastroenterology of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China.

Experimental & Molecular Medicine
|October 20, 2022
PubMed
Summary
This summary is machine-generated.

Discover the latest RNA modification detection technologies for studying the epitranscriptome. This review categorizes methods like sequencing and nanopore technologies, aiding functional epitranscriptomic research.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • Over 170 RNA chemical modifications regulate gene expression.
  • This regulatory layer is known as the epitranscriptome.
  • Advanced detection methods are crucial for epitranscriptome research.

Purpose of the Study:

  • To review current RNA modification detection technologies.
  • To categorize existing detection methods.
  • To discuss challenges and future directions in epitranscriptome analysis.

Main Methods:

  • Categorization of RNA modification detection technologies based on throughput and principles.
  • Review of quantification, locus-specific, next-generation sequencing (NGS), and nanopore direct RNA sequencing methods.

Main Results:

  • Identified four main classes of RNA modification detection technologies.
  • Summarized the principles and applications of each class.
  • Highlighted limitations and areas for improvement in current tools.

Conclusions:

  • Comprehensive understanding of the epitranscriptome requires robust detection tools.
  • Technological advancements are essential for functional epitranscriptomic studies.
  • Future research should focus on overcoming existing challenges in RNA modification detection.