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Related Experiment Videos

Heparin as antibacterial agent in rabbit bladder.

P M Hanno, R Fritz, A J Wein

    Urology
    |October 1, 1978
    PubMed
    Summary

    The rabbit bladder

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    Area of Science:

    • Urology
    • Microbiology
    • Biochemistry

    Background:

    • The rabbit bladder's primary defense against bacteria involves the antiadsorptive properties of its surface mucopolysaccharide layer.
    • Disruption of this mucopolysaccharide layer increases bacterial adhesion to the bladder surface.

    Purpose of the Study:

    • To investigate the role of mucopolysaccharides in bladder antibacterial defense.
    • To evaluate the efficacy of heparin as an antiadherence factor.

    Main Methods:

    • Experimental instillation of heparin into rabbit bladders.
    • Assessment of bacterial adsorption to bladder surfaces with and without mucopolysaccharide layers.
    • Inhibition studies using protamine to block heparin's effect.

    Main Results:

    • Heparin instillation prevented increased bacterial adsorption on denuded bladder surfaces.
    • Protamine inhibited the protective effect of heparin, confirming the mucopolysaccharide nature of the antiadherence factor.
    • Direct application of small heparin amounts significantly reduced bacterial adsorption on deficient bladders and bacteria.

    Conclusions:

    • Surface mucopolysaccharides are crucial for the rabbit bladder's antiadherence mechanism.
    • Heparin effectively mimics this natural defense, preventing bacterial adhesion.
    • Findings support mucopolysaccharides as a therapeutic target for preventing bladder infections.

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