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Nanomaterial-assisted CRISPR gene-engineering - A hallmark for triple-negative breast cancer therapeutics advancement

Jabeen Farheen1,2, Narayan S Hosmane3, Ruibo Zhao1,2,4

  • 1Institute of Smart Biomedical Materials, School of Materials Science and Engineering, Zhejiang Sci-Tech University, Hangzhou, 310018, PR China.

Materials Today. Bio
|October 21, 2022
PubMed

Insights

Triple-negative breast cancer (TNBC) requires advanced treatments. This review explores CRISPR-nano complexes for TNBC gene therapy, focusing on delivery, toxicity, and clinical applications.

Area of Science:

  • Oncology
  • Genomics
  • Biotechnology

Background:

  • Triple-negative breast cancer (TNBC) is an aggressive malignancy with poor prognosis and high metastatic potential.
  • Current treatments are limited, necessitating novel therapeutic strategies.
  • CRISPR-Cas gene editing combined with nanotechnology offers a promising avenue for cancer treatment.

Purpose of the Study:

  • To detail the genetic mechanisms underlying TNBC development.
  • To review current and emerging gene therapies for TNBC.
  • To provide an overview of CRISPR-nano complex delivery systems and applications in TNBC.
  • To discuss recent advances and toxicity concerns of CRISPR-nano complexes in TNBC clinical trials.

Main Methods:

  • Comprehensive literature review of TNBC genetics, current therapies, and CRISPR-nano technology.
  • Analysis of gene editing mechanisms and delivery systems for TNBC.
  • Evaluation of recent clinical trial data and toxicity profiles of CRISPR-nano complexes.

Main Results:

  • TNBC exhibits frequent rare mutations contributing to its aggressive nature.
  • CRISPR-nano complexes show potential for targeted gene manipulation in TNBC.
  • Challenges in delivery, biodegradability, and toxicity of CRISPR-nano complexes need to be addressed for clinical translation.

Conclusions:

  • CRISPR-nano technology holds significant promise for advancing TNBC treatment through precise gene editing.
  • Further research is crucial to optimize delivery systems and mitigate toxicity for safe and effective clinical application in TNBC.
Keywords:
CRISPR/Cas9Gene therapyGenetic mechanismNanocarriersTriple-negative breast cancer

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