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Dog secretin: sequence and biologic activity.

Y Shinomura, J Eng, R S Yalow

    Life Sciences
    |September 7, 1987
    PubMed
    Summary
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    Researchers purified dog secretin, a hormone crucial for digestion. They discovered its amino acid sequence and found it effectively stimulates pancreatic ductal flow, similar to pig secretin.

    Area of Science:

    • Gastroenterology
    • Endocrinology
    • Biochemistry

    Background:

    • Secretin is a vital gastrointestinal hormone regulating pancreatic and biliary secretions.
    • Understanding species-specific variations in secretin structure and function is important for comparative physiology.

    Purpose of the Study:

    • To purify and determine the amino acid sequence of dog secretin.
    • To compare the biological potency of dog secretin with pig secretin in stimulating pancreatic ductal flow.

    Main Methods:

    • Purification of secretin from canine intestinal tissue using acid-ethanol extraction, acetone precipitation, Sephadex gel filtration, and High-Performance Liquid Chromatography (HPLC).
    • Amino acid sequencing of purified dog secretin.
    • Bioassay in rats to assess the potency of dog secretin in stimulating pancreatic ductal flow.

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    Main Results:

    • Dog secretin was successfully purified from canine proximal intestine.
    • The amino acid sequence of dog secretin was determined as HSDGTFTSELSRLRESARLQRLLQGLV.
    • Dog secretin demonstrated equal potency to pig secretin in stimulating pancreatic ductal flow in a rat bioassay.

    Conclusions:

    • The study successfully elucidated the primary structure of dog secretin.
    • Dog secretin exhibits functional similarity to pig secretin, particularly in its role in pancreatic secretion.
    • The identified amino acid substitution at position 15 (Glu for Asp) does not alter its biological potency compared to pig secretin.