Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A freeze-etching study on experimental murine mastitis.

K Smith, R L Chandler

    Veterinary Pathology
    |September 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Adjunctive therapy in epilepsy: a cost-effectiveness comparison of two AEDs.

    Seizure·1999
    Same author

    Traumatic rupture of the aortic arch treated by stent grafting.

    European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery·1999
    Same author

    Characteristics of protein and energy metabolism in neonates with necrotizing enterocolitis--a pilot study.

    Journal of pediatric surgery·1999
    Same author

    Children's Defense Fund focuses Congress on child care: both sides of the aisle submit proposals.

    Pediatric nursing·1999
    Same author

    Electrophysiological evidence of cortical fusion in children with early-onset esotropia.

    Investigative ophthalmology & visual science·1999
    Same author

    Adenosine and pediatric supraventricular tachycardia in the emergency department: multicenter study and review.

    Annals of emergency medicine·1999

    Streptococcal mastitis research in mice shows bacteria can enter mammary epithelial cells. However, cell junctions act as a barrier, limiting bacterial spread through intercellular spaces.

    Area of Science:

    • Microbiology
    • Pathology
    • Electron Microscopy

    Background:

    • Mastitis is a significant concern in mammary gland health.
    • Streptococcal infections are a common cause of mastitis.
    • Understanding bacterial invasion mechanisms is crucial for treatment.

    Purpose of the Study:

    • To investigate the cellular pathway of *Streptococcus* invasion in experimentally induced mastitis.
    • To evaluate the role of epithelial junctional complexes as a barrier to bacterial spread.

    Main Methods:

    • Experimental induction of streptococcal mastitis in mice.
    • Intramammary inoculation with *Streptococcus* bacteria.
    • Electron microscopy, including freeze-etching and ultrathin sectioning, of affected mammary gland tissue.

    Related Experiment Videos

    Main Results:

    • Inoculated *Streptococcus* were observed within the acinar lumen, phagocytes, and epithelial cells.
    • No significant pathological changes were detected in the junctional complexes between secretory epithelial cells.
    • Bacteria demonstrated potential to move from the lumen into the epithelium and towards subepithelial areas.

    Conclusions:

    • Epithelial junctional complexes represent a potential barrier to intercellular bacterial movement during mastitis.
    • Electron microscopy provides detailed insights into bacterial-host cell interactions in mammary glands.
    • Further research may explore strategies to enhance these epithelial barriers.