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Subcellular localization and interactions among TGB proteins of cowpea mild mottle virus

S L Carvalho1, J Tilsner2,3, A R Figueira4

  • 1Departamento de Fitopatologia, Universidade Federal de Viçosa, Av. P. H. Rolfs, s/ nº Campus Universitário, 36570-000, Viçosa, MG, Brasil.

Archives of Virology
|October 21, 2022
PubMed

Insights

Cowpea mild mottle virus (CPMMV) movement proteins share similarities with potex-like viruses. Interactions between CPMMV and Potato Virus X movement proteins were analyzed, revealing cross-species effects on subcellular localization.

Area of Science:

  • Plant Virology
  • Molecular Plant Pathology
  • Virus-Host Interactions

Background:

  • Cowpea mild mottle virus (CPMMV) is a Carlavirus known to infect various legumes.
  • The triple gene block (TGB) of CPMMV, encoded by ORFs 2-4, is crucial for viral movement.
  • Understanding TGB protein function and interactions is key to deciphering viral spread.

Purpose of the Study:

  • To investigate the subcellular localization and interactions of CPMMV TGB proteins.
  • To explore potential functional complementation between CPMMV and Potato Virus X (PVX) TGB proteins.

Main Methods:

  • Transient expression of CPMMV TGB proteins fused to fluorophores.
  • Analysis of subcellular localization patterns of individual and interacting TGB proteins.
  • Assessing cross-species interactions between CPMMV and PVX TGB proteins.

Main Results:

  • CPMMV TGB proteins exhibit similar accumulation patterns and interactions to those of potex-like viruses.
  • PVX TGBp2 and TGBp3 influenced the localization of CPMMV TGBp1 to plasmodesmata.
  • The reciprocal interaction, where CPMMV TGB proteins affected PVX TGB protein localization, was not observed.

Conclusions:

  • CPMMV TGB proteins function similarly to their potex-like counterparts.
  • Evidence of functional cross-complementation exists between CPMMV and PVX TGB proteins.
  • Specific PVX TGB proteins can mediate the plasmodesmal targeting of a CPMMV TGB protein, suggesting conserved mechanisms in viral cell-to-cell movement.
Keywords:
cowpea mild mottle virussubcellular localizationtriple gene block

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