Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-Molecule Detection Concepts Enabled by DNA Origami.

Micromachines·2026
Same author

Surface Plasmon Resonance as a Potential Diagnostic Tool for the Detection of CXC Chemokine Receptor 4 (CXCR4) on Extracellular Vesicles.

Biosensors·2026
Same author

SpermFACS: Validation of a Highly Sensitive Sperm Cell Sorting Method for Sexual Assault Casework Analysis.

Analytical chemistry·2026
Same author

HematoCARD: A Volumetric Duplicate Dried Blood Spot Collection Cartridge Validated for Therapeutic Drug Monitoring.

Analytical chemistry·2025
Same author

Label-Free Rapid Quantification of Abscisic Acid in Xylem Sap Samples Using Surface Plasmon Resonance.

Biosensors·2025
Same author

Multiplex and Spatiotemporal Detection of Single-Cell Antibody Secretions Using Protein-Patterned Microwells.

Analytical chemistry·2025

Related Experiment Video

Updated: Aug 24, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

1.6K

Design and Implementation of a Dual-Region Self-Referencing Fiber-Optic Surface Plasmon Resonance Biosensor.

Valentina Bello1,2, Wouter Vandezande3, Devin Daems2

  • 1Department of Electrical, Computer and Biomedical Engineering, University of Pavia, 27100 Pavia, Italy.

ACS Sensors
|October 21, 2022
PubMed
Summary

This study introduces a dual-region fiber-optic biosensor for accurate biomolecular detection. It uses a reference channel to correct for environmental changes, improving real-time analysis of binding events.

Keywords:
aptamer-based bioassaydual-region biosensorfiber-optic biosensorhigh-resolution melting assaysurface plasmon resonance

More Related Videos

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
09:23

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Published on: December 13, 2017

6.4K
A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response
09:03

A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response

Published on: January 7, 2019

7.2K

Related Experiment Videos

Last Updated: Aug 24, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

1.6K
Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
09:23

Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

Published on: December 13, 2017

6.4K
A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response
09:03

A Silicon-tipped Fiber-optic Sensing Platform with High Resolution and Fast Response

Published on: January 7, 2019

7.2K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Optics and Photonics

Background:

  • Self-referencing is crucial for accurate biosensing.
  • External factors like temperature and refractive index changes can interfere with measurements.
  • Fiber-optic surface plasmon resonance (SPR) biosensors offer high sensitivity but require compensation for environmental variations.

Purpose of the Study:

  • To design and validate a dual-region self-referencing fiber-optic SPR biosensor.
  • To enable simultaneous measurement of binding events and environmental parameters.
  • To improve the reliability and accuracy of biosensing applications.

Main Methods:

  • Theoretical simulation of biosensor probe performance using numerical modeling.
  • Experimental validation in sucrose-water solutions to determine sensitivity to refractive index and temperature.
  • Implementation of an aptamer-based bioassay and a high-resolution melting assay.

Main Results:

  • Two distinct biosensor probe configurations were investigated.
  • Sensitivities up to 2000 nm/RIU for refractive index and 1.559 nm/°C for temperature were achieved.
  • Successful demonstration of simultaneous analysis of binding events and external signal modifications.

Conclusions:

  • The developed dual-region self-referencing biosensor effectively compensates for external variations.
  • This technology enhances the accuracy and feasibility of real-time biomolecular interaction analysis.
  • The biosensor probe is suitable for various bioassays requiring precise environmental monitoring.