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Exosome-like nanovesicles derived from Phellinus linteus inhibit Mical2 expression through cross-kingdom regulation

Jingxia Han1,2, Ting Wu1,2, Jing Jin1

  • 1Cheermore Cosmetic Dermatology Laboratory, Shanghai, China.

Journal of Nanobiotechnology
|October 22, 2022
PubMed
Abstract

Insights

Phellinus linteus (PL) derived nanovesicles containing miR-CM1 show anti-aging effects by inhibiting Mical2 expression in skin cells. This cross-kingdom regulation protects against UV-induced skin aging.

Area of Science:

  • Mycology
  • Nanotechnology
  • Molecular Biology

Background:

  • Phellinus linteus (PL) is a medicinal fungus with known antitumor and anti-inflammatory properties.
  • Research on the anti-photoaging effects of PL and fungal exosome-like nanovesicles (FELNVs) is limited.
  • Exosome-like nanovesicles, containing miRNAs, are recognized for their cross-kingdom regulatory functions.

Purpose of the Study:

  • To evaluate the anti-aging effects of Phellinus linteus (PL).
  • To isolate and characterize fungal exosome-like nanovesicles (FELNVs) from PL.
  • To elucidate the molecular mechanisms underlying the anti-aging activity of PL FELNVs.

Main Methods:

  • Isolation and characterization of FELNVs from PL.
  • In vitro analysis of FELNVs' effects on skin cells (HaCaT).
  • Assessment of molecular markers for skin aging, including Mical2, COL1A2, MMP1, ROS, MDA, SA-β-Gal, and SOD activity.
  • Volunteer testing to confirm anti-aging effects.

Main Results:

  • PL demonstrated anti-aging activity in volunteer testing.
  • Isolated FELNVs are nanoparticles (100-260 nm) with a double shell structure.
  • miR-CM1 within FELNVs inhibited Mical2 expression in HaCaT cells, promoted COL1A2, inhibited MMP1, reduced ROS/MDA/SA-β-Gal, and increased SOD activity, counteracting UV-induced damage.

Conclusions:

  • Fungal exosome-like nanovesicles (FELNVs) are key functional components of Phellinus linteus (PL).
  • miR-CM1, delivered via FELNVs, inhibits Mical2 expression in human skin cells through cross-species regulation.
  • This mechanism effectively mitigates UV-induced skin aging.
Keywords:
Anti-aging effectsCross-kingdom regulationsFungi exosome-like nanovesiclesSkin agingmiRNAs

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