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Whole-Mount In Situ Hybridization (WISH) for Salamander Embryos and Larvae

Sruthi Purushothaman1, Ashley W Seifert2

  • 1Department of Biology, University of Kentucky, Lexington, KY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 22, 2022
PubMed

Keywords:
DigoxigeninIn situ hybridization (ISH)In vitro transcriptionLimb developmentLimb regenerationRiboprobeSalamander

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In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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