Soil to earthworm bioaccumulation of polyhalogenated carbazoles and related compounds: Lab and field tests

Polyhalogenated carbazoles (PHCZs) are an emerging group of organic contaminants that have attracted attention because of their ubiquity, resistance to biodegradation, and toxicities. However, studies on the bioaccumulation of PHCZs in terrestrial organisms are limited. In the present study, bioaccumulation of 11 PHCZs and two related compounds, carbazole (CZ) and benzocarbazole (BZCZ), from soil to earthworms was investigated by paired soil-earthworm samples from Hangzhou, China and a laboratory bioaccumulation test. The sum of the concentrations of the 11 polyhalogenated carbazoles (Σ₁₁PHCZs), CZ and BZCZ in soils from Hangzhou were 1.78-67.27 ng/g dry weight, 1.11-57.90 ng/g dry weight, and 22.87-171.98 ng/g dry weight, respectively, while those in the earthworms were 179.49-892.90 ng/g lipid weight, 42.90-2140.42 ng/g lipid weight, and not detectable-2514.76 ng/g lipid weight, respectively. The average in situ biota-to-soil accumulation factors (BSAFs) ranged from 0.38 to 13.23, comparable to those in some reports for polychlorinated biphenyls and polybrominated diphenlethers. Site-independence of BSAFs and no correlation between log C_worm and log C_soil together support the hypothesis that distribution of PHCZs between soil and worms in Hangzhou didn't reach equilibrium. In the laboratory test, the accumulation trends of CZ, BZCZ, 3-bromocarbazole, 3,6-dichlorocarbazole, and 2,7-dibromocarbazole well fit to the first-order kinetics, with r² ranging from 0.796 to 0.997. The BSAFs under two exposure concentration groups at steady-state conditions were 38.8-56.0 and 2.1-4.4, respectively, suggesting the capacity of bioaccumulation for these compounds. Enhancement of concentrations and resident time of the chemicals in soil would reduce the BSAF values, which may be related to the change of uptake process of the compound or redistribution of compound between soil and earthworm. A comparison of the theoretical steady-state concentrations with the nonlinear regression-based concentrations indicates that increasing the exposure time beyond 28 days is beneficial for studying the bioaccumulation of PHCZs.