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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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Related Experiment Video

Updated: Aug 24, 2025

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
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Thermodynamic database supports deciphering protein-nucleic acid interactions.

Long-Can Mei1, Ge-Fei Hao2, Guang-Fu Yang1

  • 1Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China.

Trends in Biotechnology
|October 22, 2022
PubMed
Summary
This summary is machine-generated.

Understanding protein-nucleic acid interactions (PNIs) is key to molecular recognition. The Protein-Nucleic Acid Thermodynamics Database (PNATDB) provides crucial thermodynamic data for PNI research, freely accessible online.

Keywords:
binding affinitybioinformaticsdata setprotein–nucleic acid interactionstherapeutic oligonucleotides

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Bioinformatics

Background:

  • Protein-nucleic acid interactions (PNIs) are fundamental to cellular processes.
  • Understanding the thermodynamics of PNIs is essential for deciphering molecular recognition mechanisms and associated pathologies.

Purpose of the Study:

  • To introduce the Protein-Nucleic Acid Thermodynamics Database (PNATDB).
  • To provide a centralized resource for experimentally determined thermodynamic parameters of PNIs.

Main Methods:

  • Data compilation from experimentally determined thermodynamic studies.
  • Integration of sequence, structural, and functional data relevant to PNIs.

Main Results:

  • The PNATDB contains a comprehensive collection of thermodynamic data for PNIs.
  • The database includes associated biological context, enhancing its utility.

Conclusions:

  • The PNATDB serves as a valuable, free online resource for researchers studying PNIs.
  • This database facilitates advancements in understanding molecular recognition and related diseases.