Effects of the application timing of anti-erosive agents on dentin erosion

This in vitro study aimed to evaluate the effects of the application timing of anti-erosive agents on dentin erosion. Eighty dentin specimens with dimensions of 2 × 2 × 2 mm were prepared and randomly divided into 4 groups based on the treatment solutions: 1.23 × 10⁴ μg/ml sodium fluoride (NaF), 120 μg/ml chlorhexidine (CHX), 300 μg/ml quercetin (QUE), and deionized water (DW, negative control). The specimens in each group were further divided into 2 subgroups according to the application timing of the treatment solutions (n = 10): before the erosive challenges (PRE) and after the erosive challenges (POST). All specimens were submitted to 4 daily erosive challenges for 5 d. For each erosive challenge, the specimens in the subgroup PRE were treated with the respective solutions for 2 min and then immersed in cola drinks for 5 min, while the specimens in the subgroup POST were immersed in cola drinks for 5 min followed by treatment with the respective solutions for 2 min. The erosive dentin loss (EDL) was measured using a contact profilometer, and the surface morphology of the dentin specimens was evaluated by scanning electron microscopy at the end of the experiment. The data were statistically analyzed using two-way analysis of variance (ANOVA) and Bonferroni's test (α = 0.05). Significantly less EDL was observed in the groups NaF, CHX, and QUE than in the group DW (all P < 0.001). Significantly lower EDL was observed in the groups CHX and QUE than in the group NaF (P = 0.001 and P < 0.001, respectively). For CHX, subgroup POST exhibited significantly less EDL than subgroup PRE (P < 0.001). Regarding QUE, subgroup PRE showed significantly less EDL than subgroup POST (P < 0.001). Furthermore, a relatively greater number of obliterated dentinal tubules was visible in the subgroup POST rather than in the subgroup PRE of the group CHX, while in the group QUE, narrower dentinal tubules were observed in the subgroup PRE than those in subgroup POST. In conclusion, CHX and QUE showed the best performance in controlling dentin erosion. CHX was more effective in reducing EDL when applied after erosive challenges, whereas QUE worked more effectively when used before erosive attacks. The application timing should be considered when evaluating the effects of anti-erosive agents because it may determine their effectiveness.