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Updated: Aug 24, 2025
Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Published on: April 12, 2019
LIMS2 is Downregulated in Osteosarcoma and Inhibits Cell Growth and Migration
Chenying Su1, Xiaona Cai2, Taotao Xu3
1Department of Traditional Chinese Medicine Orthopedics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China.
Methods:
GEO, GEPIA, and UALCAN databases were used to assess LIMS2 expression in OS. UALCAN and CCLE databases were applied to assess the methylation levels of LIMS2 in OS tissues and cells, which was verified in OS cells using the methylation specific PCR. The effects of LIMS2 on regulating OS cell growth, migration and invasion were determined by CCK-8, Edu staining, and transwell chambers, respectively. The role of LIMS2 in the activation of MAPK signaling was assessed using western blotting assay in OS cells.
Results:
LIMS2 expression was declined in OS tissues and cells, while its methylation level was increased. The low expression of LIMS2 was associated with shorter overall survival and disease-free survival. Overexpression of LIMS2 inhibited cell growth, migration, and invasion and decreased the levels of p-ERK/ERK, p-P38/P38, and p-JNK/JNK.
Conclusion:
LIMS2 expression was decreased in OS, which was associated with hypermethylation level and poor prognosis. LIMS2 overexpression inhibited OS cell growth and migration, which may be caused by the suppression of MAPK signaling.
Insights
LIMS2 expression is decreased in osteosarcoma (OS) due to hypermethylation, correlating with poor prognosis. LIMS2 overexpression inhibits OS cell growth and migration by suppressing MAPK signaling.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Osteosarcoma (OS) is a primary bone malignancy with poor prognosis.
- Understanding the molecular mechanisms underlying OS progression is crucial for developing effective therapies.
Purpose of the Study:
- To investigate the role of LIM and SH3 domain-containing protein 2 (LIMS2) in osteosarcoma (OS).
- To determine the correlation between LIMS2 expression, methylation, and patient survival.
- To elucidate the functional impact of LIMS2 on OS cell behavior and signaling pathways.
Main Methods:
- Bioinformatic analysis using GEO, GEPIA, and UALCAN databases for LIMS2 expression and methylation.
- Methylation-specific PCR to validate LIMS2 methylation in OS cells.
- In vitro assays (CCK-8, Edu staining, Transwell) to assess LIMS2's effect on cell growth, migration, and invasion.
- Western blotting to evaluate LIMS2's impact on MAPK signaling pathway activation.
Main Results:
- LIMS2 expression was significantly decreased in OS tissues and cells.
- Increased LIMS2 methylation levels were observed in OS, correlating with decreased expression.
- Low LIMS2 expression was associated with shorter overall survival and disease-free survival.
- Overexpression of LIMS2 suppressed OS cell proliferation, migration, and invasion.
- LIMS2 overexpression led to decreased phosphorylation of ERK, P38, and JNK in MAPK signaling.
Conclusions:
- LIMS2 is downregulated in osteosarcoma, linked to hypermethylation and adverse prognosis.
- LIMS2 functions as a tumor suppressor in OS by inhibiting cell growth and migration.
- The tumor-suppressive role of LIMS2 may be mediated through the inhibition of the MAPK signaling pathway.
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