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Related Experiment Video

Updated: Aug 24, 2025

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
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TGF-β controls stromal telomere length through epigenetic modifications.

Rajeev Mishra1,2, Subhash Haldar3, Shea Biondi2

  • 1Department of Life Sciences and Biotechnology, Chhatrapati Shahu Ji Maharaj University, Kalyanpur, Kanpur, UP 208024 India.

3 Biotech
|October 24, 2022
PubMed
Summary

Transforming growth factor-beta (TGF-β) regulates prostate stromal cell telomere length by increasing Suv39h1 expression. Notch and androgen signaling also cooperate with TGF-β in this process, offering new therapeutic targets for prostate cancer.

Keywords:
CancerEpigeneticsTGF-βTelomere lengthTumor microenvironment

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Area of Science:

  • Epigenetics
  • Cancer Biology
  • Molecular Oncology

Background:

  • Telomere length is crucial for cellular function and is epigenetically regulated.
  • Altered telomere length in cancer and stromal cells is linked to prostate cancer progression and metastasis.
  • Transforming growth factor-beta (TGF-β) signaling influences prostate tumorigenesis, but its role in stromal telomere regulation is unclear.

Purpose of the Study:

  • To investigate the role of TGF-β in regulating telomere length in prostate fibroblasts.
  • To elucidate the molecular mechanisms underlying TGF-β-mediated telomere regulation.
  • To explore the interplay between TGF-β, Notch, and androgen signaling in stromal telomere maintenance.

Main Methods:

  • Utilized real-time reverse transcription-polymerase chain reaction (RT-PCR) for telomere length measurement.
  • Assessed the expression of histone methyltransferase Suv39h1.
  • Investigated the effects of DAPT (Notch inhibitor) and bicalutamide (antiandrogen) on stromal telomere length.

Main Results:

  • TGF-β was found to regulate telomere length in mouse and human prostate fibroblasts.
  • TGF-β increased the expression of Suv39h1, affecting histone methylation at telomeric ends.
  • Notch and androgen signaling pathways were shown to cooperate with TGF-β in regulating stromal telomere length.

Conclusions:

  • TGF-β plays a significant role in regulating prostate stromal cell telomere length via Suv39h1-mediated epigenetic modifications.
  • The findings highlight a novel mechanism involving TGF-β, Notch, and androgen signaling in prostate cancer progression.
  • Understanding stromal telomere regulation offers promising avenues for prostate cancer diagnosis, prognosis, and therapeutic development.