Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

2.7K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
2.7K
Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

4.9K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy of PD-1/PD-L1 and LAG-3 immune checkpoint inhibitors in the treatment of patients with solid tumor.

Frontiers in immunology·2026
Same author

An Implantable Optogenetics-Engineered Hydrogel for Amelioration of Rheumatoid Arthritis through Light-Controlled Metabolic Reprogramming of Synovial Macrophages.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

From Concept to Clinic: Innovative Applications and Design Evolution of Microneedles in Wound Healing.

Advanced healthcare materials·2026
Same author

Retraction Note: Adipose tissue-derived stem cells suppress hypertrophic scar fibrosis via the p38/MAPK signaling pathway.

Stem cell research & therapy·2026
Same author

Emerging strategies in senotherapeutics: from broad-spectrum senolysis to precision reprogramming.

npj aging·2026
Same author

The impact of obesity or overweight on the risk of glaucoma: a meta-analysis.

Frontiers in medicine·2026

Related Experiment Video

Updated: Aug 24, 2025

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
11:00

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Published on: January 12, 2015

11.7K

Adipose-Derived Stem Cell Exosomes Inhibit Hypertrophic Scaring Formation by Regulating Th17/Treg Cell Balance.

Hongtao Wang1, Yan Li1, Zhang Yue1

  • 1Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.

Biomed Research International
|October 24, 2022
PubMed
Summary
This summary is machine-generated.

Adipose-derived stem cell-derived exosomes (ADSC-Exos) modulate T cell differentiation, reducing inflammatory factors and promoting regulatory T cells (Treg). This suggests a therapeutic role for ADSC-Exos in managing hypertrophic scarring.

More Related Videos

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate
11:31

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate

Published on: October 31, 2012

16.0K
Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells ASCs
08:52

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells ASCs

Published on: August 11, 2016

7.0K

Related Experiment Videos

Last Updated: Aug 24, 2025

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
11:00

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Published on: January 12, 2015

11.7K
Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate
11:31

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate

Published on: October 31, 2012

16.0K
Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells ASCs
08:52

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells ASCs

Published on: August 11, 2016

7.0K

Area of Science:

  • Immunology
  • Regenerative Medicine
  • Dermatology

Background:

  • Hypertrophic scarring involves dysregulated inflammation and extracellular matrix deposition.
  • Th17 cells and regulatory T cells (Treg) play critical roles in scar formation and maturation.
  • The therapeutic potential of adipose-derived stem cell-derived exosomes (ADSC-Exos) in scar modulation is under investigation.

Purpose of the Study:

  • To investigate the role of ADSC-Exos in modulating Th17 and Treg cell differentiation.
  • To determine the effect of ADSC-Exos on inflammatory factor secretion and extracellular matrix deposition in hypertrophic scarring.
  • To explore the therapeutic potential of ADSC-Exos for hypertrophic scar treatment.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA), qRT-PCR, and immunoblotting were used to quantify inflammatory factors (IL-6, IL-10, IL-17A, TNF-α) and transcription factors (RORγt, Foxp3).
  • Immunohistochemistry staining and immunoblotting assessed extracellular matrix deposition.
  • Experiments were conducted both in vitro using naïve CD4+ T cells and in vivo using mouse wound models treated with ADSC-Exos.

Main Results:

  • Hypertrophic scars showed elevated levels of IL-6, IL-10, IL-17A, TNF-α, RORγt, and Foxp3 compared to normal skin.
  • ADSC-Exos treatment in vitro reduced IL-6, IL-17A, TNF-α, and RORγt while increasing IL-10 and Foxp3 expression in naïve CD4+ T cells.
  • Mice treated with ADSC-Exos exhibited reduced collagen deposition, decreased IL-17A, TNF-α, and RORγt, and increased IL-10 and Foxp3.

Conclusions:

  • ADSC-Exos effectively modulate T cell differentiation towards a Treg phenotype and away from Th17.
  • ADSC-Exos treatment reduces key inflammatory mediators and extracellular matrix deposition associated with hypertrophic scarring.
  • ADSC-Exos demonstrate significant therapeutic potential for managing and treating hypertrophic scars.