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Updated: Aug 24, 2025

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
Published on: July 1, 2014
Residue-specific N-terminal glycine to aldehyde transformation renders analytically pure single-site labeled proteins
Tularam Sahu1, Mohan Kumar1, Sajeev T K2
1Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhauri, Bhopal, MP 462 066, India. vrai@iiserb.ac.in.
Abstract:
Here, we present N-Gly-specific glyoxamide generation in native proteins, isolated or in a complex mixture. The resulting aldehyde enables parallel installation of probes and a purification platform to render analytically pure single-site tagged proteins. It renders N-Gly engineered insulin without perturbing its structure, receptor binding, and downstream signaling pathway.
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