Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CD74 ablation rescues type 2 diabetes mellitus-induced cardiac remodeling and contractile dysfunction through pyroptosis-evoked regulation of ferroptosis.

Pharmacological research·2022
Same author

Activation of the NLRP3 inflammasome by RAC1 mediates a new mechanism in diabetic nephropathy.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]·2022
Same author

Optimal regenerative repair of large segmental bone defect in a goat model with osteoinductive calcium phosphate bioceramic implants.

Bioactive materials·2022
Same author

Development of Cell-Carrying Magnetic Microrobots with Bioactive Nanostructured Titanate Surface for Enhanced Cell Adhesion.

Micromachines·2021
Same author

Deficiency of IKK<i>α</i> in Macrophages Mitigates Fibrosis Progression in the Kidney after Renal Ischemia-Reperfusion Injury.

Journal of immunology research·2021
Same author

Multi-Modal Image Fusion Based on Matrix Product State of Tensor.

Frontiers in neurorobotics·2021

Related Experiment Video

Updated: Aug 24, 2025

Bone Marrow-derived Macrophage Production
07:06

Bone Marrow-derived Macrophage Production

Published on: November 22, 2013

73.8K

Producing Genetically Engineered Macrophages With Enhanced Immunity via Microinjection.

Yang Jiao, Fei Pan, Shuxun Chen

    IEEE Transactions on Nanobioscience
    |October 24, 2022
    PubMed
    Summary

    Microinjection offers an efficient and safe method for genetically engineering immune cells, overcoming limitations of traditional gene delivery. This technique successfully enhanced macrophage immunity by overexpressing the toll-like receptor 4 (Tlr4) gene.

    More Related Videos

    Production and Characterization of Human Macrophages from Pluripotent Stem Cells
    08:05

    Production and Characterization of Human Macrophages from Pluripotent Stem Cells

    Published on: April 16, 2020

    16.7K
    Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages
    12:47

    Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

    Published on: November 3, 2014

    11.7K

    Related Experiment Videos

    Last Updated: Aug 24, 2025

    Bone Marrow-derived Macrophage Production
    07:06

    Bone Marrow-derived Macrophage Production

    Published on: November 22, 2013

    73.8K
    Production and Characterization of Human Macrophages from Pluripotent Stem Cells
    08:05

    Production and Characterization of Human Macrophages from Pluripotent Stem Cells

    Published on: April 16, 2020

    16.7K
    Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages
    12:47

    Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

    Published on: November 3, 2014

    11.7K

    Area of Science:

    • Immunology and Genetics
    • Cell Biology and Gene Delivery

    Background:

    • Conventional gene delivery methods like lentivirus and cationic lipid transfection exhibit low efficiency and high toxicity in immune cells.
    • Macrophages are crucial immune cells, but efficient genetic modification remains a challenge.

    Purpose of the Study:

    • To develop a novel, high-throughput microinjection system for efficient gene delivery into macrophages.
    • To create a genetically engineered macrophage cell line with enhanced immunity via microinjection of the toll-like receptor 4 (Tlr4) gene.

    Main Methods:

    • Development of a home-made, high-throughput microinjection system.
    • Microinjection of a Tlr4 expression plasmid into the Raw264.7 mouse macrophage cell line.
    • Quantification of gene expression using enhanced green fluorescent protein (eGFP) fusion and assessment of mRNA and protein levels.

    Main Results:

    • Achieved a 90% gene expression efficiency in microinjected Raw264.7 cells.
    • Demonstrated remarkable up-regulation of Tlr4 mRNA and protein expression.
    • Observed enhanced expression of Tlr4 downstream genes and improved inhibition of tumor cell migration and invasion in engineered macrophages.

    Conclusions:

    • Microinjection is an efficient, safe, and high-throughput method for genetic engineering of macrophages.
    • Overexpression of Tlr4 in macrophages enhances their immune function and anti-tumor capabilities.
    • This method overcomes limitations of traditional transfection, avoiding polarization effects.