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Fighting multidrug-resistant Enterococcus faecalis via interfering with virulence factors using green synthesized
Abdullah Yousef1, Mohammed Abu-Elghait1, Mohammed G Barghoth1
1Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, 11884, Cairo, Egypt.
Microbial Pathogenesis
|October 24, 2022
Summary
Green synthesized silver nanoparticles (Ag-NPs) effectively reduce virulence factors in multidrug-resistant Enterococcus faecalis without impacting cell growth. This offers a promising strategy against antibiotic resistance.
Area of Science:
- Nanotechnology
- Microbiology
- Materials Science
Background:
- Multidrug resistance in Enterococcus faecalis poses a significant global health threat.
- Virulence factors contribute to the pathogenicity and persistence of E. faecalis infections.
- Novel therapeutic strategies are urgently needed to combat resistant bacterial strains.
Purpose of the Study:
- To synthesize silver nanoparticles (Ag-NPs) using a green method.
- To evaluate the efficacy of green synthesized Ag-NPs against virulence factors of multidrug-resistant E. faecalis.
- To characterize the synthesized Ag-NPs.
Main Methods:
- Green synthesis of Ag-NPs.
- Determination of E. faecalis virulence factors and selection of potent isolates.
- Characterization of Ag-NPs using UV-Visible spectroscopy, FTIR spectroscopy, Dynamic Light Scattering (DLS), and Transmission Electron Microscopy (TEM).
- Assessment of Ag-NPs' effect on planktonic cell growth and virulence factors.
Main Results:
- Green synthesized Ag-NPs were characterized, showing a spherical shape with an average size of 28.8 nm.
- UV-Visible spectroscopy revealed surface Plasmon resonance (SPR) bands between 256-345 nm and 510 nm.
- A concentration of 0.0625 mg/ml Ag-NPs significantly reduced virulence factors in multidrug-resistant E. faecalis without affecting planktonic cell growth.
- DLS indicated a tendency towards electrostatic attraction between nanoparticles in the solution.
Conclusions:
- Green synthesized Ag-NPs are effective in combating multidrug-resistant E. faecalis by inhibiting virulence factors.
- This approach presents a viable strategy to address the growing crisis of antimicrobial resistance.
- Further research into Ag-NPs as an alternative therapeutic agent is warranted.
Keywords:
Enterococcus faecalisGreen synthesisNanoparticlesQuorum sensing system -multi-drug resistant bacteriaVirulence factorsMore Related Videos
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