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Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

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Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
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Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
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Updated: Aug 24, 2025

Generation of Murine Monoclonal Antibodies by Hybridoma Technology
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Functional antibody delivery: Advances in cellular manipulation.

Kayla C Koch1, Gregory N Tew2

  • 1Department of Polymer Science & Engineering, University of Massachusetts, Amherst, MA 01003, United States.

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|October 24, 2022
PubMed
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Intracellular antibody delivery is crucial for expanding therapeutic targets beyond the cell surface. This review explores methods for delivering functional antibodies into cells to control cellular functions.

Keywords:
ApoptosisCell differentiationCell growthCell motilityCell signalingMembrane transductionProtein expression

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Immunology

Background:

  • The therapeutic antibody market is rapidly growing, with current therapies targeting extracellular proteins.
  • Antibodies cannot naturally cross cell membranes, limiting their therapeutic applications to extracellular targets.
  • Intracellular targets offer vast potential for novel therapeutic strategies.

Purpose of the Study:

  • To review the emerging field of functional intracellular antibody delivery.
  • To highlight the necessity of delivering antibodies into the cytosol for cellular manipulation.
  • To discuss the potential of intracellular antibodies for controlling cell function.

Main Methods:

  • Review of existing literature on antibody delivery vehicles (e.g., nanoparticles, cell-penetrating polymers).
  • Focus on studies measuring functional cellular responses post-delivery.
  • Analysis of antibodies designed for intracellular targets like proteins and DNA.

Main Results:

  • Various delivery systems show promise for model antibody cargo.
  • A limited number of studies successfully demonstrate functional antibody delivery and measure cellular response.
  • Intracellularly delivered antibodies can be engineered to control specific cellular functions.

Conclusions:

  • Functional intracellular antibody delivery is essential for advancing cellular manipulation and expanding therapeutic targets.
  • Further research is needed to optimize delivery systems and validate cellular responses.
  • This approach holds significant promise for novel therapeutic interventions.