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Updated: Aug 24, 2025

Electron Cryotomography of Bacterial Cells
Published on: May 6, 2010
Structural investigation of eukaryotic cells: From the periphery to the interior by cryo-electron tomography
Chunyang Wang1, Matthias Wojtynek1, Ohad Medalia1
1Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.
Abstract:
Cryo-electron tomography (cryo-ET) combines a close-to-life preservation of the cell with high-resolution three-dimensional (3D) imaging. This allows to study the molecular architecture of the cellular landscape and provides unprecedented views on biological processes and structures. In this review we mainly focus on the application of cryo-ET to visualize and structurally characterize eukaryotic cells - from the periphery to the cellular interior. We discuss strategies that can be employed to investigate the structure of challenging targets in their cellular environment as well as the application of complimentary approaches in conjunction with cryo-ET.
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