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Evidence for non-specifically bound IgG in human tumours
Acta Pathologica Et Microbiologica Scandinavica. Section C, Immunology
|October 1, 1978
Summary
Immunoglobulin G (IgG) was found in all malignant human tumors tested, with most eluting at 56°C. Some IgG was non-specifically bound, as indicated by marker antibodies in tumor eluates.
Area of Science:
- Oncology
- Immunology
- Biochemistry
Background:
- Immunoglobulin G (IgG) can be found in human tissues.
- Non-specific binding of IgG can complicate analysis.
Purpose of the Study:
- To investigate the presence and binding characteristics of IgG in malignant human tumors.
- To differentiate between specifically and non-specifically bound IgG in tumor tissues.
Main Methods:
- Homogenized human malignant and normal tissues were analyzed.
- Continuous flow elution technique was used to prepare heat eluates.
- IgG antibodies to rabbit erythrocytes served as marker antibodies for non-specifically bound IgG.
Main Results:
- All 13 malignant tumors tested contained IgG, with elution primarily at 56°C, but also at 37°C and 45°C.
- Marker antibodies indicated non-specific IgG binding in 4 tumors.
- Normal organs generally contained minimal IgG, with slightly higher amounts in kidneys from older individuals.
Conclusions:
- Malignant human tumors consistently contain IgG.
- A portion of IgG found in tumors is non-specifically bound.
- Further research is needed to understand the specific role of tumor-associated IgG.