Challenging the water stress index concept: Thermographic assessment of Arabidopsis transpiration

Water stress may greatly limit plant functionality and growth. Stomatal closure and consequently reduced transpiration are considered as early and sensitive plant responses to drought and salinity stress. An important consequence of stomatal closure under water stress is the rise of leaf temperature (T_leaf ), yet T_leaf is not only fluctuating with stomatal closure. It is regulated by several plant parameters and environmental factors. Thermal imaging and different stress indices, incorporating actual leaf/crop temperature and reference temperatures, were developed in previous studies toward normalizing for effects unassociated to water stress on T_leaf , aiming at a more efficient water stress assessment. The concept of stress indices has not been extensively studied on the model plant Arabidopsis thaliana. Therefore, the aim of this study was to examine the different indices employed in previous studies in assessing rosette transpiration rate (E) in Arabidopsis plants grown under two different light environments and subjected to salinity. After salinity imposition, E was gravimetrically quantified, and thermal imaging was employed to quantify rosette (T_rosette ) and artificial reference temperature (T_wet, T_dry ). T_rosette and several water stress indices were tested for their relation to E. Among the microclimatic growth conditions tested, RWSI₁ ([T_rosette  - T_wet ]/[T_dry  - T_wet ]) and RWSI₂ ([T_dry  - T_rosette ]/[T_dry  - T_wet ]) were well linearly-related to E, irrespective of the light environment, while the sole use of either T_wet or T_dry in different combinations with T_rosette returned less accurate results. This study provides evidence that selected combinations of T_rosette , T_dry , and T_wet can be utilized to assess E under water stress irrespective of the light environment.