Streptomyces solincola sp. nov., isolated from soil in Malaysia

A urease-producing Gram-stain-positive actinobacterium, designated strain T5^T, was isolated from a soil sample collected at a highway hillslope in Selangor, Malaysia. The strain was found to produce pale yellowish-pink aerial mycelia with smooth long chain spores and extensively branched light yellowish-pink substrate mycelia on oatmeal agar. Strain T5^T grew at 15-37 °C, pH 6-11, and tolerated up to 9 % (w/v) NaCl, with optimal growth occurring at 28 °C, pH 6-9 and without NaCl. The whole-cell sugar hydrolysate of strain T5^T contained galactose, glucose and ribose. The ll-diaminopimelic acid isomer was detected in the cell wall. Diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were found to be the predominant polar lipids. The main fatty acids were anteiso-C_17 : 0, iso-C_16 : 0, anteiso-C_15 : 0 and iso-C_14 : 0. Comparative analysis of the 16S rRNA gene sequences indicated that strain T5^T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01^T (99.0 %). The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01^T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization. Phylogeny analyses showed that strain T5^T was closely related to Streptomyces fradiae, Streptomyces lavendofoliae, Streptomyces lichenis, Streptomyces roseolilacinus and Streptomyces somaliensis. Based on these polyphasic data, strain T5^T represents a novel species, for which the name Streptomyces solincola sp. nov. is proposed. The type strain is T5^T (=TBRC 5137^T= DSM 42166^T).